久久久久人妻精品一区三寸,成年免费视频黄网站在线观看

TranslationMobile

表達克隆[通過進行基因表達而克隆目的基因的方法
表達克隆化

"expression" in Chinese : n. 1.表現(xiàn)，表示，表達。 2.詞句；語句，措辭，說法 ...
"clone" in Chinese : n. 1.【生物學(xué)】純種細胞，無性系。 2.復(fù)制品；(幾 ...
"cloning by expression" in Chinese : 表達克隆
"expression cloning phenotype cloning" in Chinese : 表達克隆化
"cloning" in Chinese : 基因轉(zhuǎn)殖克隆; 克隆繁殖; 克隆技術(shù); 克盧克隆化; 無性繁殖系化; 無 ...
"cloning of" in Chinese : n基因的克隆
"expression" in Chinese : n. 1.表現(xiàn)，表示，表達。 2.詞句；語句，措辭，說法。 3.表情，臉色， ...
"no expression" in Chinese : 沒有感情（表情）
"the expression" in Chinese : 一詞
"no expression no expression" in Chinese : 呆板的表情，冰冷的聲音; 沒有表情
"antibody cloning" in Chinese : 抗體（基因）克隆; 抗體克隆
"car cloning" in Chinese : 汽車復(fù)制
"cdna cloning" in Chinese : cdna克隆化
"clone；cloning" in Chinese : 基因轉(zhuǎn)殖
"cloning (programming)" in Chinese : 克隆 (信息學(xué))

Examples

In our study we have cloned the osd gene from s . typhimurium by pcr , characterized the gene product and used this gene to construct asd + expression cloning vectors ptrc99a - asd
再將hpylori尿素酶b亞單位基因與尿素酶b和熱休克蛋白a融合基因分別克隆入ptrc99a一asd質(zhì)粒的多克隆位點之內(nèi)。
Conclusion constructed the high - level expression clone of echistatin in e . coli . the expression of recombinant protein is higher , it make the further study of echistatin feasible
結(jié)論成功構(gòu)建了echistatin的原核高效表達克隆，表達量高于現(xiàn)有國內(nèi)外研究水平，為echistatin功能及相關(guān)疾病的研究奠定了基礎(chǔ)。
In the first part , the focus is to find the receptor molecules directly by screening two cdna libraries with a recombinant construct prpap or as an alternative , to find an enriched area in the embryo brains and construct libraries from this brain region and perform the expression cloning as above
方法：（）以融合蛋白prpap通過瞬時表達克隆法篩選兩個cdna文庫，或者通過與胚胎腦的結(jié)合實驗篩選有豐富結(jié)合蛋白的腦區(qū)，以圖構(gòu)建cdna文庫并進行表達克隆的篩選。
Objective : construct high - level expression system of echistatin in e . coli methods : obtain amino - acid sequence of echistatin from genebank database . considering the bias of usage of 61 available aminoacid codons in e . coli , design the coding sequence of echistatin , synthesize the dna sequence chemically , get single copy coding gene and repeated two copy coding gene of echistatin . insert the sequence into expression vector pbv220 , and more , we construct fusion expression clone of echistatin with pcr , identify the recombinant vector by dna sequencing
目的構(gòu)建蛇毒鋸鱗蝰素( echistatin )的原核高效表達體系方法由genebank數(shù)據(jù)庫檢索蛇毒鋸鱗蝰素( echistatin )的氨基酸序列，結(jié)合大腸桿菌蛋白質(zhì)合成體系對氨基酸密碼子使用的偏愛性，設(shè)計了echistatin編碼基因，體外人工合成編碼基因dna片段，通過適當(dāng)?shù)南拗菩詢?nèi)切酶位點插入表達載體pbv220 ，分別構(gòu)建了echistatin的單拷貝表達克隆、雙拷貝串聯(lián)表達克隆；進一步通過pcr技術(shù)構(gòu)建echistatin的融合表達基因克隆。